Product   Laboratory results Comparisons
Immune Pro versus other whey proteins
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Please note that in the attachment below (provided by the manufacturer of Immune ProŽ in response to our request), and on the company's website, there is NO COMPARATIVE DATA offered to substantiate the claims of ImmuneoPro's superiority over other whey proteins.
In fact, the attachment claims that the analysis of ImmunePro is an "Analysis of 80% Whey Protein Concentrate", contrary to the claims on their website. Aditionally, the concentration and percentages of ImmunePro's bioactive ingredients (lactoferrin, immunoglobulins, and bovine serum albumin) are less than those published for Immunocal Bioactive Whey Protein as substantiated by Immunocal Bioactive Whey Protein Patent #5,888,552.



-----Original Message-----
From: LYoss@aol.com
To: contact@immunepro.com
Sent: Saturday, September 30, 2000 11:08 AM
Subject: More Information Please!

> I would be interested in seeing the clinical trials done on ImmunePro Rx to
> substantiate your claim that this product is more potent than other whey
> protein products. I have read your entire website and can find no information...
> Where is your proof? Testimonials
> (only one could I find) are anecdotal, and do not support or prove such a
> claim. Any medical doctor would know that. Who is Carol Seiverling, and
> what are her credentials?
> Thank you for your anticipated prompt reply to my questions.
> Rayne Yoss


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From: Wellsprings [mailto:contact@immunepro.com]
Sent: Saturday, September 30, 2000 2:45 PM
To: LYoss@aol.com
Subject: Re: More Information Please!

> The unique quality of our new product is substantiated by fact, the published analysis.
> We welcome our competition to do the same.
>The benefits of whey are now being documented from many published doctors.
>Results from the clinical trial are forthcoming.
>Wellsprings


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----- Original Message -----
From: Steven P. Petrosino
To: contact@immunepro.com
Sent: Saturday, September 30, 2000 10:40 AM
Subject: Request for comparative information


> Greetings:
> I noted on your website that a WPI typically contains 90% protein, however
> your Nutrition Facts product labeling claims 4 grams of protein per 5 gram
> serving. This is 80% protein by weight (4/5 grams=80%), not 90%. Can you
> address this for me?
> Also, please send me detailed information on your product to include total
> calories, fat content, cholesterol content, Lactose content, protein
> content, and total sugars. I would also be interested in any laboratory
> comparisons you have to support your claims of superior bioactivity versus
> ImmuPlus, Optimune, and [Immunocal Bioactive Whey Protein].

> Thank you.
> Regards,
> Steven P. Petrosino
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>-----Original Message-----
>From: Wellsprings [mailto:contact@immunepro.com]
>Sent: Saturday, September 30, 2000 2:35 PM
>To: Steven P. Petrosino
>Subject: Re: Request for comparative information


>Greetings,
>The FAQ and our product page info in our web site answers most of your questions.
>The Dr David Marshall paper is very detailed on whey production. WPI/WPC.
>ImmunePro Rx is many times more potent than any dietary whey product anywhere. We publish our analysis, file attached.
>We welcome our competition to do the same.
>If you phone our office on a week day Michael Keenan can answer all of your detailed questions.
>Wellsprings


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ATTACHMENT
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"Analysis of 80% Whey Protein Concentrate by SDS PAGE Gel Method


ImmuneProŽ
Lactoferrin
3.4%
Immunoglobulins
16.1
Bovine Serum Albumin
2.5
beta-lactoglobulin
48.0
alpha-lactalbumin
13.7


Sample Preparation: The samples were diluted in sample buffer containing 5.0% sodium dodecyl sulfate (SDS), 10% glycerol, and 63 mM tris, pH 6.8. The tubes were placed in a boiling water bath for 10 minutes to ensure dissolution.

SDS slab gel electrophoresis was carried out according to the method of Laemmli (Laemmeli, U.K. Nature 227: 680-685, 1970) as described by O'Farrell (J. Biol. Chem. 250:4007-4021,1975, second dimension) using a 10% acrylamide slab gel (125 mm length X 150 mm width X 0.75 mm thickness) overlaid with a 25 mm stacking gel. The following proteins (Sigma Chemical Co., St. Luois, MO) were added to molecular weight standards to weigh eight: myosin (220,000), phosphorylase A (94,000), catalase (60,000), actin (43,000), carbonic anhydrase (29,000), and lysozyme (14,000). Electrophoresis was carried out using 12 mAmp/gel for about 3.5 hours at which time the bromophenol blue front had migrated to the end of the slab gel. The gel was stained with Coomassie blue dye, destained in 10% acetic acid until a clear background was obtained, and dried between cellophane sheets.

December 7, 1999"