Dr. Petrosino's Biography Directions
Ghrelin and weight loss


My Story

01-06-99 As a clinical scientist and a certified nutritionist, I probably would have never tried Calorad® if it had not been recommended to me by my best friend, Scott.

01-10-99 Purchased and started using Calorad® for the first time.

01-17-99 Day 7 - Weight loss ...4 lbs.

01-24-99 Day 14 - I lose 4 more lbs and decide to become a distributor!

02-10-99 Day 30 - I finish my first bottle and lose .. another 4 lbs for a total of 12 pounds!

02-10-99 Day 30 I have lost almost 12 pounds and over two and a half inches off my waist within my first four weeks on Calorad®.

02-10-99 Day 30 My wife, Lynn, loses three pounds and a total of five inches in the same time period.

02-24-99 Six weeks on the product. I experience increased energy, improved sleep, and several lipofuscin deposits (age spots) on my hands recede and totally disappear.

03-03-99 My wife, who previously suffered from frequent and rather severe bouts of insomnia, now 'sleeps like a baby.'

04-07-99 My teenage son and daughter also start to use the product and experience similar weight loss and muscle toning.

05-11-99 My sister loses 10 pounds and two dress sizes in three weeks, and she loves the product.

11-07-99 I decide to spread the word online, and establish my nutrition advisor website.

As a clinical scientist with a doctorate in nutrition, I can truly say that Calorad® is one of the best diet products I've ever seen!

In the 13 years since, we have sold over $4.5 million dollars of Calorad® online, and have seen many great Calorad® success stories.

Sincerely, Steven P. Petrosino, Lt. Col. USMC (Retired)

Order Calorad® Now at our sale price!


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A New Obesity Related Peptide

Ghrelin, an endogenous ligand for growth hormone seretagogue  receptor (GHS-Rs), regulates pituitary growth hormone (GH) secretion. GHS-Rs distributed on hypothalamic neurons and in the brainstem. Ghrelin participates in  energy balance in rodents by decreasing fat utilization without significantly changing energy expenditure or locomotor activity. Peripheral daily administration of Ghrelin caused weight gain (obesity) by reducing fat utilization in mice and rats. Intracerebroventricular administration of Ghrelin generated a dose-dependent increase in food intake and body weight. Rat serum Ghrelin concentrations were increased by fasting and were reduced by re-feeding or oral glucose administration, but not by water ingestion. Ghrelin as a brain-gut peptide, in addition to its role in regulating GH secretion, enable to involve in regulation of food intake and body weight. A gut hormone designated as PYY (Peptide YY(3-36) (PYY(3-36)), a Y2R agonist) is one of the most potent orexigenic substances yet to be identified. PYY is released from the gastrointestinal tract postprandially in proportion to the calorie content of a meal. Information on PYY is found HERE

Tschop M, et al. Nature, 407, 908~913 (2000)

Hosoda, H, et al. J Biol Chem (2000 May 8)

Kojima, M, et al. Nature 402,656-660 (1999)

Wren A.M. et al, Endocrinology 141: 4325-4328, 2000

More references on Ghrelin


Motilin-Related Peptide

A new gut peptide, is specifically expressed in enteroendocrine cells of the stomach. 1). The amino acid sequence of human Prepro- Motilin- Related Peptide/ m46 protein is completely identical with human prepro-Ghrelin  except Ser26 is not octanoylated. 2). The amino acid sequence of rat Pro-Motilin-Related Peptide/m46 Protein is also completely identical with rat Pro-Ghrelin except Ser26 is not octanoylated. 3). Human Motilin-Related Peptide/m46 Protein (24-41) is identical with human Des- octanoyl3- Ghrelin(1-18). 4). Rat and mouse Motilin-Related Peptide/m46 Protein (24-41) is also identical with rat Des-octanoyl3-Ghrelin

Tomasetto C., et al. Gasenterology 119, 395-405 (2000)

Kojima, M, et al. Nature 402,656-660 (1999)

Review by Dr, Jaw-Kang Chang

Please visit Motilin-Related Peptide Page!





Intravenous injection of Ghrelin (Rat), Ghrelin (Human) stimulates growth hormone release

Anti-Ghrelin (Rat) Antibody (H-031-31) works on IHC mapping (1:70)

Normal plasma level of human Ghrelin = 87.79±10.27 pg/ml (Mean ± SE, n=7)*

Normal plasma level of Rat Ghrelin =      74.87 ± 8.22 pg/ml (Mean ± SE, n=10)*

Dr. C Y  Bowers, School of Medicine, Tulane University Medical Center

* Plasma was extracted using C18 column

Peptide % Crossreactivity 
Ghrelin (Rat) 100
Ghrelin (Human) 100
Ghrelin (23-28) (Human, Rat) 26.7
Ghrelin (1-14) (Des-Octanoyl3) (Human) 0
Ghrelin (1-5) (Des-Octanyl3) (Human, Rat) 0
Secretin (Human) 0
PrRP31(Rat) 0
PrRP31 (Human) 0
Galanin (Rat) 0
Galanin (Human) 0
GHRF (Rat) 0
GHRF (Human) 0
NPY (Human, Rat) 0
Orexin A 0
Orexin B (Human) 0
Orexin B (Mouse, Rat) 0


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DISCLAIMER: We make no medical claims regarding our supplements. Our nutritional supplements are not intended to treat or cure any disease. Please contact your physician before embarking on any weight loss or exercise program.

This site was modified to present format on August 5, 2004.
This website was originally Established on November, 7 1997.